Figure 3
Total anthocyanin content of grapevine cultures under different lighting conditions and at different time points. All cultures originated from the same source culture. All 26 replicate cultures were cultivated for 4 weeks at 26 °C, shaking at 140 rpm. Every week, 15% of the cells (v/v) were subcultured and the remaining cells were collected for LC-IMS-HRMS analysis to determine anthocyanin concentrations. Each line represents a lighting condition: conditions 1–24 are based on the DoE model (Table 1) with an intensity of red + green + blue light = 50 µmol m2 s 1 and UV light either off or on for 1 h (12–1 pm) each day (9 µmol m2 s1). Those cultures were cultivated in the LiS under 24 different lighting conditions with a 16-h photoperiod. The light control was cultivated according to the conditions of the routine/source culture (standard shaking incubator, light positioned in the ceiling, 16-h photoperiod, 80 µmol m2 s1), but was contaminated after 3 weeks, therefore the data represent only 2 weeks of cultivation. The dark control was cultivated in the absence of light. There seemed to be an error with sample preparation or analysis of culture 17 in week 2, but we decided to keep the point in the graph in order to not delete any data.
