Table 6 Predicted and actual ratios between the anthocyanin concentrations of cells cultivated under different lighting conditions.

From: LEDitSHAKE: a lighting system to optimize the secondary metabolite content of plant cell suspension cultures

Goal

Predicted ratio (optimized condition/light control) week 2

Actual ratio (optimized condition/light control) week 2

Goal achieved?

Pn-Glu up

2.1

1.8

Yes, but prediction not reached

Cy-Glu up

1.6

2.4

Yes, better than prediction

Dp-Glu down

0.6

0.5

Yes, better than prediction

Pt-Glu down

0.7

0.3

Yes, better than prediction

  1. Predicted ratios were calculated the following before conducting the control experiment: the anthocyanin concentration in week 2 in cells cultivated under the optimized lighting conditions were predicted using the numerical optimization tool of the Design-Expert software and then divided by the actual anthocyanin concentration in cells cultivated under white light in week 2 of the main experiment. Actual ratios were calculated after analyzing the control experiment: the actual anthocyanin concentration in cells cultivated under optimized lighting conditions in week 2 of the control experiment was divided by the actual anthocyanin concentration in cells cultivated under white light in week 2 of the control experiment.
  2. Conc. = Concentration, Cy = Cyanidin, Dp = Delphinidin, Pn = Peonidin, Pt = Petunidin, Glu = Glucoside, Up = Upregulation (the goal is to maximize the concentration), Down = Downregulation (the goal is to minimize the concentration).
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