Figure 4

Contractile element stiffness (\({{k}}_{{{{CE}}}}\)). Trial specific \(k_{CE}\) values were inferred from a 3-parameter fit (Eq. 1), with information from one oscillation period after TD (Supplementary Fig. S1). \(k_{CE}\) is also given as scaled to the dimension of one representative half-sarcomere (\(k_{hs}\), right axis, see Eq. 2). The correspondingly scaled isometric force per half-sarcomere (Eq. 3) is given on the upper axis. The solid and dashed grey lines represent the model1 and model2 fits (see Table 2), respectively. The dotted, horizontal, black line indicates the median of passive \(k_{CE}\) values. The thin, black line underlying the model2 fit, is the model1 fit with only the parameter \(\Delta L_{CE}\) open (fixed \(C_{fil}\,=\,0.0067\,\hbox {nm}\,\hbox {pN}^{-1}\)).