Figure 1 | Scientific Reports

Figure 1

From: Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells

Figure 1

Production and characterisation of NOD.Va14tg mice. (A) A diagram of the transgenic construct, which consists of a CD4 minigene, including a minimal CD4 promoter/enhancer and the CD4 intronic silencer 5’ of Vα14-Jα18 TCRα chain cDNA and a poly-A tail17. (B) Pups were genotyped by using construct-specific primers. mwm = molecular weight marker; ntc = no template control. Results are shown for nine individual transgenic mice from NOD.Va14tg Line 1. (C) Thymic, hepatic and splenic NKT cell phenotyping of NOD.Va14tg Lines 1–4 transgenic mice compared to WT NOD mice (n = 10: Data means (± SEM) from 5 males and 5 females for each group. Data are combined from two independent experiments and NKT gating is indicted at the top for WT NOD mice).

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