Figure 3
Comparison of hepatic and splenic NKT cell subsets and cytokine production in WT and NOD.Va14tg mice. A) Representative FACS plots (left panel) show the mean frequencies (± SEM) of NKT cell subsets in total hepatic (Top panels) or splenic (Bottom panels) NKT cells of NOD WT and NOD.Va14tg mice. (A) CD4/CD8 NKT cell subsets. (B) CD24/CD44 NKT cell subsets. (C) CD44/NK1.1 NKT cell subsets. (D) CD4/NK1.1 NKT cell subsets. Histograms (right panel) show the mean frequencies and absolute numbers of hepatic or splenic NKT cell subsets between NOD WT (clear bars) and the four NOD.Va14tg mouse lines (filled bars). (n = 10 female mice/group. Data are combined from two independent experiments). (B) The effect of i.v. α-GalCer administration on cytokine production in NOD.Va14tg mice shows that Vα14 transgenic expression increased cytokine production. Histograms show the mean concentration (± SEM) of IL-4, IFN-γ, IL-6, IL-2, GM-CSF, TNF and IL-1 in serum of NOD WT mice (clear bars) and NOD.Va14Tg/1 mice (filled bars), 4 h after challenge with α-GalCer (n = 6–10 mice/group).
