Figure 2

Comparison of the aggregation of peptides with 13 alanine repeats under multiple concentrations, temperatures, and incubation times. (a) Quantification of the area of aggregate clusters of TAMRA-labeled 13A peptide (100 µg/mL, red) incubated for 4 h at room temperature (RT) (n = 15 from 7 images), for 2 d at 37 °C (n = 15 from 9 images), for 7 d at 37 °C (n = 14 from 7 images), or for 7 d at 50 °C (n = 16 from 6 images) (bottom). Representative confocal images are shown (top). ANOVA followed by posthoc test, *p < 0.05, **p < 0.01. (b) TAMRA-labeled 13A peptide (1 mg/mL, red) incubated for 4 h at RT or for 7 d at 37 °C. Representative confocal images are shown (left). Quantification of the percentage of the area occupied by aggregates in a field of view (n = 5 each) (right). Each error bar represents the SE. Student’s t-test, **p < 0.01. Scale bars, 50 µm.