Table 2 SARS-CoV-2 inactivation by rapid antigen test buffers.
From: Virucidal efficacy of guanidine-free inactivants and rapid test buffers against SARS-CoV-2
Product name (manufacturer) | Active ingredient/s, if known* | Purification resin‡ | Reagent: virus ratio | Contact time (min) | Titre reduction, log10 TCID50/ml [± 95% CI)†| Virus detectable in TCID50 (virus detected in n/N replicates) |
|---|---|---|---|---|---|---|
ESPLINE SARS-CoV-2 extraction solution (Fujirebio) |  ≥ 0.25– ≤ 0.5% cetrimonium chloride | SM2 | 2.3:1 | 1 | 0.2 [− 0.3–0.6] | Yes (3/3) |
5 | 0.4 [− 0.1–0.8] | Yes (3/3) | ||||
10 | 0.2 [− 0.2–0.6] | Yes (3/3) | ||||
Panbio COVID-19 Ag rapid test device buffer (Abbott) | 0.49% Tween-20 | PDRR | 3:1 | 1 | 0.2 [− 0.3–0.6] | Yes (3/3) |
5 | 0.6 [0.1–1.0] | Yes (3/3) | ||||
10 | 0.2 [− 0.2–0.7] | Yes (3/3) | ||||
Healgen coronavirus Ag rapid test cassette swab buffer (Zhejiang Orient Gene) | Not known | PDRR | 3:1 | 1 | 0.8 [0.4–1.2] | Yes (3/3) |
5 | 1.4 [1.0–1.8] | Yes (3/3) | ||||
10 | 1.6 [1.2–2.0] | Yes (3/3) | ||||
LumiraDx SARS-CoV-2 Ag test extraction buffer (LumiraDx) | 0.5% unspecified detergent 1.00% Tween 20 | PDRR | 7.5:1 | 1 |  − 0.4 [− 0.9–0.0] | Yes (3/3) |
5 |  − 0.5 [− 0.9– − 0.1] | Yes (3/3) | ||||
10 |  − 0.5 [− 1.0–0.0] | Yes (3/3) | ||||
COVID-19 (SARS-CoV-2) antigen test kit extraction reagent (Anhui Deepblue Medical Technology) | Not known | None | 3:1 | 1 | 0.0 [− 0.4–0.3] | Yes (3/3) |
5 | 0.0 [− 0.6–03] | Yes (3/3) | ||||
10 | 0.1 [− 0.3–0.5] | Yes (3/3) | ||||
Veritor extraction reagent (BD) | 0.1– < 1% Triton X-100 | PDRR | 3.25:1 | 1 |  ≥ 4.5 [4.2–4.8] | No3 |
5 |  ≥ 5.4 [5.2–5.7] | No2 | ||||
10 |  ≥ 5.7 [5.4–6.0] | No1 | ||||
SARS-CoV-2 quick antigen extraction buffer (ScheBo) |  < 1% Triton X-100 | PDRR | 2.5:1 | 1 | 5.3 [5.1–5.6] | Yes (3/3) |
5 |  ≥ 6.0 [5.7–6.3] | Yes (2/3) | ||||
10 |  ≥ 6.0 [5.7–6.3] | No4 | ||||
Vstrip COVID-19 antigen rapid test extraction buffer (Panion & BF Biotech) |  < 1% Tergitol < 1.2% Methanol | PDRR | 10:1 | 1 | 0.1 [− 0.3–0.4] | Yes (3/3) |
10 | 0.0 [− 0.5–0.3] | Yes (3/3) | ||||
SARS-CoV-2 antigen qualitative test extraction solution (Innova) | Not known | None | 2:1 | 1 | 0.1 [− 0.3–0.5] | Yes (3/3) |
5 | 0.1 [− 0.2–0.5] | Yes (3/3) | ||||
10 | 0.0 [− 0.5–0.4] | Yes (3/3) | ||||
COVID-19 rapid antigen test sample buffer (Mologic) | 0.4% unspecified surfactant | PDRR | 3.5:1 | 1 |  ≥ 5.4 [5.1–5.7] | No1 |
Extraction buffer from Standard Q COVID-19 Ag Test Kit (SD Biosensor) | Not known | PDRR | 2:1 | 1 | 1.7 [1.3–2.1] | Yes (3/3) |
5 | 2.4 [2.0–2.8] | Yes (3/3) | ||||
10 | 2.9 [2.4–3.3] | Yes (3/3) | ||||
Extraction buffer from Standard Q COVID-19 Ag Saliva test kit (SD biosensor) | Not known | PDRR | 3.5:1 | 1 | 0.8 [0.4–1.2] | Yes (3/3) |
5 | 1.1 [0.7–1.6] | Yes (3/3) | ||||
10 | 1.8 [1.4–2.2] | Yes (3/3) |
