Figure 5 | Scientific Reports

Figure 5

From: CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations

Figure 5

In vitro Screening of non-responsive TATAloxP with T > C mutations. (A) Schematic representation of the in vitro recombination assay with TATAloxP sequences. (B,C) Linear DNAs with different TATAloxP sequences containing the indicated mutations were incubated with Cre recombinase. Note that TATAloxP with the T > C mutation at the first base probably did not efficiently produce recombined DNA in the first experiment because of technical errors (B), but did so very well in the second experiment (C). (NC; No loxP, PC; positive control DNA in Cre recombinase (NEB Catlog# M0298S)).

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