Figure 3 | Scientific Reports

Figure 3

From: Temperature responsive chromatography for therapeutic drug monitoring with an aqueous mobile phase

Figure 3

Chromatograms of hydrophobic steroids using crosslinked PNIPAAm modified-bead packed columns using beads prepared via polymerization for (A) 5 h, and (B) 18 h. The mobile phase was pure water. The flow rate of the mobile phase was 1.0 mL/min. Detection at 254 nm. Peak 1 = hydrocortisone, peak 2 = prednisolone, peak 3 = dexamethasone, peak 4 = hydrocortisone acetate, and peak 5 = testosterone.

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