Figure 5 | Scientific Reports

Figure 5

From: Validation of reference genes for quantitative real-time PCR in chemical exposed and at different age’s brackish water flea Diaphanosoma celebensis

Figure 5

Relative quantification of (A) GSTsigma and (B) EcRA expression in D. celebensis at different ages, 24 h, 4 d, 7 d, and 10 d. The error bars represent the standard errors of the means of three biological replicates. Different letters and asterisks indicate significantly different expressions compared to the 24 h sample (ANOVA, p < 0.05) and differences under different normalizations of the reference gene at expression levels for the same age, respectively (t-test, p < 0.05).

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