Table 1 Summary of nine reference genes.
Gene symbol | Gene description | GenBank accession no | Primer sequences (5′-3′) | Amplicon size (bp) | Tm (°C) | Efficiency (%) |
|---|---|---|---|---|---|---|
Atb | Alpha-tubulin | MH636293 | GCATGATTTCCAACACGAC TACCAGTGAACGAAGGCT | 97 | 55.2 | 93.92 |
53.9 | ||||||
Act | Beta-actin | MH636289 | CAAGATTGTCGCTCCTCCTG CATCTGCTGGAAGGTGG | 84 | 60.5 | 90.79 |
59.5 | ||||||
18 s | 18 s ribosomal RNA | AF144210.1 | TGGAAGGATTGACAGATTGA CTTAGTTGGTGGAGCGATTT | 81 | 54.3 | 101.89 |
56.4 | ||||||
GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | MH636290 | AACTGTCGCCGCTGTTGA ATGGAATGTTCTTGGGGTCG | 86 | 56.1 | 96.31 |
58.4 | ||||||
EF-1b | Elongation factor 1-beta | MH636295 | CGGCTGTGTCGTTGAAGA GGCAATGTCCACACTCTG | 94 | 56.1 | 106.67 |
56.1 | ||||||
UBC | Ubiqutin Conjugating enzyme | MH636294 | CCTTTTGACGGACCCATAT TAGTCCAACAGCGAGCAATA | 104 | 55.2 | 103.89 |
56.4 | ||||||
H2A | Histone H2A | MH636296 | GAATACCTGGCTGCTGAAG CAATTGGAGATGACGGGG | 90 | 56.1 | 101.35 |
57.3 | ||||||
TBP | TATA-box binding protein | MH636292 | TTTCCTGGCTTAATTTACCGTA ATCTCTTGTCTGACTTTGGC | 101 | 54.3 | 96.37 |
56.4 | ||||||
SDH | Succinate dehydrogenase | MH636291 | CATCGAGTCTCAACAGAAGA GAGCTCAACCTTTCCAGTT | 91 | 56.4 | 100.16 |
55.2 |
