Table 2 Cycle threshold (Ct) values of nine putative reference genes in 12 mRNA at different conditions (three chemicals and four concentrations) from a monoculture of Diaphanosoma celebensis exposed to chemicals.

From: Validation of reference genes for quantitative real-time PCR in chemical exposed and at different age’s brackish water flea Diaphanosoma celebensis

Gene

Ct value statistics

Min

Max

Max–Min

Mean

SD

SE

CV

Atb

21.18

22.48

1.30

21.93

0.38

0.06

0.02

Act

16.06

16.52

0.46

16.26

0.09

0.01

0.01

18S

8.70

9.61

0.91

9.19

0.21

0.03

0.02

GAPDH

17.00

18.27

1.27

17.32

0.23

0.03

0.01

EF-1b

17.34

18.39

1.05

17.80

0.20

0.03

0.01

UBC

21.40

22.23

0.83

21.82

0.20

0.03

0.01

H2A

18.26

18.98

0.72

18.49

0.17

0.02

0.01

TBP

20.85

21.65

0.8

21.21

0.20

0.03

0.01

SDH

18.60

19.54

0.94

19.00

0.19

0.03

0.01

  1. Min., minimum; Max., maximum; SD, standard deviation; SE, standard error; CV, coefficient of variation.
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