Table 3 Cycle threshold (Ct) values of nine putative reference genes in 12 mRNA samples from a monoculture of Diaphanosoma celebensis at different ages (Min., minimum; Max., maximum; SD, standard deviation; SE, standard error; CV, coefficient of variation).

From: Validation of reference genes for quantitative real-time PCR in chemical exposed and at different age’s brackish water flea Diaphanosoma celebensis

Gene

Ct value statistics

Min

Max

Max–Min

Mean

SD

SE

CV

Atb

18.85

28.27

9.42

23.82

3.41

0.57

0.14

Act

18.97

21.29

2.32

19.75

0.57

0.10

0.03

18S

9.59

13.43

3.84

11.18

1.14

0.19

0.10

GAPDH

19.41

21.79

2.38

20.31

0.63

0.11

0.03

EF-1b

21.51

24.23

2.72

22.20

0.69

0.12

0.03

UBC

24.99

26.80

1.81

25.80

0.54

0.09

0.02

H2A

22.22

23.86

1.64

22.88

0.45

0.08

0.02

TBP

24.21

26.85

2.64

25.39

0.90

0.15

0.04

SDH

22.00

24.12

2.12

22.90

0.46

0.08

0.02

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