Figure 5

Viability of A-431 cell line in response to tested compounds (TBBt, HBBH, HIIH and BIIB) evaluated with MTT metabolism assay. Cells were incubated for 72 h with increasing concentrations (range = 1.25–160 μM) of compound. Black circles represents optical density at 570 nm, blue lines shows the standard dose–response curve fitted globally to three independent experiments (all experiments are shown in Figures S13–S16), and red lines boarder the confidence bands of the fitted line. Gray points denote removed high-concentration data, for which aggregation significantly perturbs resulting viability.