Figure 3
THC Chromatogram and levels in blood and brain. (A) LC-MS Chromatogram: THC (black line) and THC-d3 (grey line) overlapping peaks at 2.7 min. (B) Blood levels: data are presented as mean ± SEM; n = 5–6 for each group. Presence of (*) indicates an administration difference with INH > INJ at 15-min at ****p < 0.0001. Presence of (#) indicates a timepoint difference with green and purple lines indicating an INH and INJ difference respectively; specifically, INH-15 > all timepoints and INH-30 > INH-60/90/240 at #p < 0.05, whereas INJ-30 > all timepoints and INJ-240 < all timepoints at ##p < 0.01. Presence of ($) indicates a sex difference; specifically, female-INH > female-INJ at $$$p < 0.001 and male-INJ > female-INJ at $$$p < 0.001). (C) Brain levels: data are presented as mean ± SEM; n = 5–6 for each group. Presence of (*) indicates an administration difference with INH > INJ at 15, 30, and 60-min at **p < 0.01. Presence of (#) indicates a timepoint difference with green and purple lines indicating an INH and INJ difference respectively; specifically, INH-15, 30, and 60 > INH-90 and 240 at ##p < 0.01, whereas INJ-90 > INJ-15 and 240 at ##p < 0.01. Presence of ($) indicates a sex difference with males > females at $$p < 0.01.
