Figure 4

11-OH-THC chromatogram and levels in blood and brain. (A) LC-MS Chromatogram: 11-OH-THC (black line) and 11-OH-THC-d3 (grey line) peaks at 2.0 min. (B) Blood levels: data are presented as mean ± SEM; n = 5–6 for each group. Presence of (*) indicates an administration difference with INH < INJ at all timepoints at *p < 0.05. Presence of (^#) indicates a timepoint difference with purple lines indicating an INJ difference where INJ-15 < INJ-30, 60, and 90 at ^#p < 0.05 and INJ-240 < INJ-30, 60, and 90 at ^###p < 0.001. Presence of (^$) indicates a sex difference; specifically, females > males at ^$p < 0.05. (C) Brain levels: data are presented as mean ± SEM; n = 5–6 for each group. Presence of (*) indicates an administration difference with INH < INJ at 30, 60, and 90-min at ***p < 0.001. Presence of (^#) indicates a timepoint difference with purple lines indicating an INJ difference where INJ-15 and 240 < INJ-30, 60, and 90 at ^###p < 0.001. Presence of (^$) indicates a sex difference; specifically, male-INH < male-INJ at ^$$$p < 0.001, female-INH < female-INJ at ^$$$$p < 0.0001, and male-INJ < female-INJ at ^$$$$p < 0.0001.