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Figure 1

From: Human organ donor-derived vagus nerve biopsies allow for well-preserved ultrastructure and high-resolution mapping of myelinated and unmyelinated fibers

Figure 1

Light micrographs of human cervical vagus nerve. (A) LM overview of a representative cervical vagus nerve from a 49 year old female. Note multiple fascicles surrounded by loose connective tissue and lipid droplets (arrows). Scale bar = 500 µm. (B, C) Detailed view of individual fascicles indicated by yellow and red boxes in (A). Note distinct perineurium forming distinct boundary of each fascicle (arrows) and presence of numerous myelinated axons within each fascicle. Scale bars = 50 µm. (D) Fascicle in (C) after segmentation of the inner and outer contours of myelinated axons (n = 345) and perineurium. Quantitative information in (E–J) are derived from contoured structures in (D). (E) Histogram of percent of fascicle area inside of perineurium occupied by myelinated axons. (F–H) Size distribution of myelinated fiber diameter, axon diameter, and myelin thickness, respectively. Note two distribution peaks suggesting two distinct populations of myelinated fibers. (I) Distribution of G-ratios for myelinated fibers. (J) Histogram of perineurium thickness ratio.

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