Figure 6 | Scientific Reports

Figure 6

From: Human organ donor-derived vagus nerve biopsies allow for well-preserved ultrastructure and high-resolution mapping of myelinated and unmyelinated fibers

Figure 6

Summary of tissue processing steps and analysis of human organ donor-derived vagus nerve samples for LM and EM studies. Please note that the tissue fixation and embedding steps of human vagus nerve tissues are shared. The tissue sectioning of embedded tissue blocks, contrasting or staining, and imaging are performed according to LM- and EM-specific protocols. Data segmentation for morphological mapping studies and quantitative analysis of fiber at both LM and EM levels are performed using same approaches, including SAE corrections of axonal size and myelination. HVN human vagus nerve, ROI region of interest, PO propylene oxide, PBS phosphate buffered saline, PF paraformaldehyde, Glut glutaraldehyde.

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