Figure 4 | Scientific Reports

Figure 4

From: Tenebrio molitor larvae meal inclusion affects hepatic proteome and apoptosis and/or autophagy of three farmed fish species

Figure 4

(a) Ubiquitin conjugates, (b) LC3BII/LC3BI ratio and (c) SQSTM1/p62 in the liver of gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax) and rainbow trout (Oncorhynchus mykiss) under the 0–25%, 0–50% and 0–60% respectively inclusion of T. molitor (TM) meal. Representative immunoblots (Western blot for LC3BII/LC3BI and SQSTM1/p62; Dot blot for ubiquitin cojuagates) are shown and were quantified by laser scanning densitometry [Gel-Pro Analyzer 4.0 software (Media Cybernetics, Inc. www.mediacy.com)] and plotted [SigmaPlot 12.5 software (Systat Software Inc. www.systatsoftware.com)]. Values represent means ± SD; n = 5 preparations from different animals. Data was statistically analyzed [GraphPad Instat 3.10 (GraphPad Software, www.graphpad.com)]: *denotes significant differences (p < 0.05) compared with 0%. The accompanying tables exhibit fold-differences between TM diet inclusion and control (0%). s, d and o denote significant differences (p < 0.05) between S. aurata, D. labrax and O. mykiss, respectively.

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