Table 1 FSP1 mRNA levels measured by real-time PCR and normalized by 18S ribosomal RNA (rRNA).

From: Intercellular interactions between mast cells and stromal fibroblasts obtained from canine cutaneous mast cell tumours

Sample

FSP1 Ct

Mean

18S Ct

Mean

2ΔΔCt

Mean

334–16 (P0)

32.61 ± 0.29

27.28 ± 0.08

93.92 ± 13.86

346–16 (P1)

23.77 ± 0.008

20.29 ± 0.19

338.46 ± 46.58

M5

23.06 ± 0.06

11.45 ± 0.02

1.20 ± 0.03

M25

23.24 ± 0.03

11.11 ± 0.007

0.83 ± 0.01

  1. Ct (cycle threshold) values to FSP1 and 18S of P0, P1, M5 and M25 cells are observed as mean ± Sd.