Figure 4
Impaired meiotic progression of Snf5 CKO female germ cells. (A,B) The chromosome spreads in E19.5 ovaries immunostained with antibodies against SYCP1 and SYCP3 (A), or with antibodies against γH2AX and SYCP3 (B). The inserts show higher-magnification views. Yellow and white arrowheads indicate synapsed and unsynapsed chromosomes, respectively. (C) Distribution of each stage of meiotic prophase. The distribution was significantly different between the control and Snf5 CKO females (**P < 0.01, χ2 test). The mean ± standard deviation is shown. Approximately 30 cells were analyzed for each mouse. (D) The percentages of γH2AX-positive pachynema (P) and diplonema (D). The percentages were significantly higher in both the pachynema and diplonema of the Snf5 CKO females (***P < 0.001, χ2 test). The number of cells analyzed are as follows: pachynema and diplonema in the control mice, 34 cells from three females and 55 cells from three females; pachynema and diplonema in the Snf5 CKO mice, 29 cells from three females and 39 cells from three females.
