Figure 2

Evaluation of BPMV detection by the RT-CPA-LFD assay. (A) Specificity of the RT-CPA-LFD assay for BPMV detection. 1–5, RNA templates of leaves infested with SBMV, ToRSV, ArMV, TRSV, and BPMV, respectively. (B–D), Sensitivity test for BPMV detection by RT-CPA-LFD, real-time fluorescent RT-CPA, and TaqMan-MGB real-time RT-PCR. Dilutions (100–10−6) of total RNA of the BPMV-infected leaf sample were with the initial concentration of 50 ng/µl. NC, negative control with RNase-free distilled water.