Figure 3

Validation of the BPMV detection methods on field samples. (A) Soybean seeds. 1–7, imported soybean seeds with suspected symptoms of BPMV infection; 8, healthy soybean seed from a local supermarket. (B–D) Detection of BPMV by RT-CPA-LFD, real-time fluorescent RT-CPA, and TaqMan-MGB real-time RT-PCR. PC, positive control of the RNA template from the BPMV-infested leaves. NC, negative control (a healthy soybean seed).