Figure 4 | Scientific Reports

Figure 4

From: Pan-AMPK activator O304 prevents gene expression changes and remobilisation of histone marks in islets of diet-induced obese mice

Figure 4

O304 mediated downregulation of Aldh1a3 is accompanied by redistribution of H3K27Ac. (a) Representative sections of HFD and HFD-O304 pancreata stained for insulin (green) and ALDH1A3 (red). (b) Quantification of the proportion of insulin positive area with immunoreactivity for ALDH1A3 in HFD (n = 8) and HFD-O304 (n = 7). ***p < 0.001 (two-tailed Student’s t test). Individual data points, mean ± SEM are indicated in all graphs. (c) Relative mRNA expression of Aldh1a3 in CBA islets cultured in vitro under conditions of low glucose (11 mM), high glucose (22 mM), and high glucose (22 mM) plus O304 (5 μM) (n = 7 for all conditions) **p < 0.01. (Wilcoxon signed rank test). (d) Read count in the Aldh1a3 locus for RNA-seq (upper panel), H3K27Ac ChIP-seq (middle panel) and H3K27Me3 ChIP-seq (lower panel) in RD (blue), HFD (red) and HFD-O304 (green) islets. Called peaks are indicated as boxes below read count. Blue shading indicates areas of differential acetylation.

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