Figure 5 | Scientific Reports

Figure 5

From: Pan-AMPK activator O304 prevents gene expression changes and remobilisation of histone marks in islets of diet-induced obese mice

Figure 5

O304 treatment reversed impaired glucose homeostasis in HFD mice. (a) Weight curve for RD (blue circles), HFD-switch (pink triangles) and O304-switch (purple squares). Dashed line indicates the timepoint for switch of diet. Fasted blood glucose (b) and insulin (c), fat mass (d) and lean mass (e) for the same mice at start of diet, at 9 weeks when diet switch was performed and at 18 weeks (from start until 9 weeks n = 9 for RD, 10 for HFD-switch, and 10 for O304-switch, thereafter 5 mice from each group were sacrificed and n = 4 for RD, 5 for HFD-switch, and 5 for O304-switch at 18 weeks). Mean ± SEM is indicated for weight curve while all other graphs display individual data points, mean ± SEM. (f) Relative mRNA expression of key islet genes in RD, HFD-switch and O304-switch islets. Significant expression changes as compared to RD controls in each of the time points are displayed with asterisks. *p < 0.05, **p < 0.01, ****p < 0.0001 (Wilcoxon rank sum test).

Back to article page