Table 1 Primers utilized in this study.

From: Molecular characterization and overexpression of the difenoconazole resistance gene CYP51 in Lasiodiplodia theobromae field isolates

Primers

Sequence (5′ → 3′)

Description

Per-1F

GCCAACAGCCACGGATGAT

Amplification of the promoter region of the Lt CYP51 gene

Per-1R

GCCATAGGTGACGGTGCTG

Lt-CYP51F

CCCTCCGTCTCCCTACACCT

Amplification of the coding region of the Lt CYP51 gene

Lt-CYP51R

TTCTCCCTCCTCTCCCAAA

RT-LtF

GGATTGTGCTTCGTCTCGC

Quantitative RT-PCR primers for analysis of Lt CYP51 expression

RT-LtR

CGTCTCCTTGACCACCTGCT

RT-Act LtF

GGAGATGAGGCACAGTCG

Amplification of the actin gene

RT-Act LtR

GCGGTGGTGGAGAAAGAGT

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