Figure 3

MicroRNA arm switching through development and evolution. (A) Heatmap showing relative arm usage changes across seven time-points of Parhyale embryonic development. Purple indicates 5' dominance, orange 3' dominance. Comparison of the relative arm usage of microRNA homologs between Parhyale and Tribolium (B), spider (C) and honeybee (D). All three show microRNAs that have undergone arm switching (5'/3' or 3'/5' quadrants). Dotted lines show the tenfold difference boundary, name labels are shown for each microRNA exceeding this tenfold change in arm usage. (E) miR-71-5p and -3p arm expression data through development for the four species analysed.