Table 1 Effect of elicitor treatments on apigenin contents.

From: Overexpression of chalcone isomerase A gene in Astragalus trigonus for stimulating apigenin

Elicitors types

Elicitors concentrations

A. trigonus Calli

A. trigonus Cell suspension

Mean apigenin content (mg/g)

Apigenin change (fold) compared with the control

Mean apigenin content (mg/g)

Apigenin change (fold) compared with the control

Control (MS free)

0

0.801 ± 0.04

1.00

0.951 ± 0.060

1.00

SA (µM)

50

0.769 ± 0.030

0.96

1.361 ± 0.300

1.43

100

0.897 ± 0.060

1.12

1.658 ± 0.200

1.74

150

0.467 ± 0.040

0.58

0.965 ± 0.040

1.01

200

0.384 ± 0.009

0.48

0.364 ± 0.030

0.38

YE (mg/l)

50

1.951 ± 0.090

2.43

2.198 ± 0.100

2.31

100

2.786 ± 0.400

3.48

3.793 ± 0.100

3.99

150

1.724 ± 0.300

2.15

2.483 ± 0.300

2.61

200

1.396 ± 0.300

1.74

1.978 ± 0.100

2.08

CH (mg/l)

50

1.860 ± 0.090

2.32

0.325 ± 0.070

0.34

100

0.191 ± 0.020

0.24

2.048 ± 0.300

2.15

150

0.101 ± 0.020

0.13

0.248 ± 0.080

0.26

200

0.654 ± 0.120

0.82

0.785 ± 0.120

0.83

JA (µM)

50

0.185 ± 0.040

0.23

0.245 ± 0.020

0.26

100

1.416 ± 0.150

1.77

2.047 ± 0.080

2.15

150

0.921 ± 0.040

1.15

2.102 ± 0.120

2.21

200

1.621 ± 0.100

2.02

1.782 ± 0.100

1.87

  1. HPLC was used for measuring the apigenin concentration in the six weeks A. trigonus calli and cell suspension cultures incubated on MS media supplemented with different concentrations of SA, YE, JA and CH.
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