Figure 6

Increased protein expression of HIF-1α downstream targets without changes in mitochondrial respiratory complexes in the heart primordium after heartbeat initiation. (A,B) Representative images of Western blots (A) and densitometry (C) of GLUT1, HK2, PFKM, GAPDH, and G6PD in the pre- and post-heartbeat groups (N = 6). (C,D) Representative images of Western blots (C) and densitometry (D) of Ser 297 phospho-PDH E1α (PDEA1), total PDEA1, PDK1, and PDP1 in the pre- and the post-heartbeat groups (N = 6). (E,F) Representative images of Western blots (E) and densitometry (F) of NDUFS1, SDHB, UQCRC2, MTCO2, and ATP5A in the pre-heartbeat group, the post-heartbeat group, and the heart primordium at E11 (N = 6). (G) Representative transmission electron microscopy images of the heart primordium in the pre-heartbeat group, the post-heartbeat group, and the heart primordium at E11. Black scale bar indicates 1.0 μm. *p < 0.05 with Welch's t-test for comparison of two groups and ANOVA with Tukey–Kramer’s post hoc test for comparison of three groups. N.S.: not significant. Full-length blots/gels are presented in Supplementary Figures S2-S4.