Figure 1

Process of the in vitro establishment and culture of H. speciosa (Gomes) cell culture. Seeds disinfected and inoculated in a tube (a). Explants obtained after 60 days of culture (b). Friable calli 120 days after the beginning of induction from leaves (c). Stable cell suspension (d). Orbital shaker used to culture the cell suspension under light support with 50 μmol m−2 s−1 irradiance (e). Bar = 1 cm.