Table 1 Primer sequences and qPCR efficiencies of the 10 candidate taimen reference genes.
From: Evaluation of qPCR reference genes for taimen (Hucho taimen) under heat stress
Accession number | Gene (Full Name) | Lift primer (5′-3′) | Right primer (5′-3′) |
|---|---|---|---|
HAGJ01000001 | 18S rRNA (18S ribosomal RNA) | CGTTCTTAGTTGGTGGAGCG | AACGCCACTTGTCCCTCTAA |
HAGJ01162991 | Saha (S-adenosyl-L-homocysteine hydrolase) | TGGAGGGATGGCTGAACATT | AGAGCACTGGAGGAAACACA |
HAGJ01029436 | GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) | GTCTTCTGGGTAGCGGTGTA | ACCATCGTCAGCAATGCATC |
HAGJ01015081 | RPL13 (ribosomal protein L13) | GGCCATCTTGAGTTCCTCCT | GCACCATTGGCATCTCTGTT |
HAGJ01017568 | ARBP (attachment region binding protein) | GGGCTTTGTCTTCACCAAGG | CTTCTCAGGACCAAGCCCAG |
HAGJ01147709 | 28S RNA (28S ribosomal RNA) | GTCCTTCTGATCGAGGCTCA | GGAGTTTACCACCCGCTTTG |
HAGJ01168534 | β-actin (actin, beta) | TCTACGAAGGCTACGCTCTG | CAGCTTCTCCTTGATGTCGC |
HAGJ01117629 | RPL19 (ribosomal protein L19) | ACACGGGCATAGGTAAGAGG | TCGATTTTCTTGGCCTCCCT |
HAGJ01023957 | RPS29 (ribosomal protein S29) | TGGGACATCAGAGCCTCTAC | CTGGCGGCACATGTTGAG |
HAGJ01091547 | α-tubulin (alpha-Tubulin) | CGAGCCATACATCACACACG | TGCAATTGGGTGTTGATCCA |
HAGJ01009947 | Hsp60 (heat shock protein 60) | GACATCATCAGACGAGCCCT | ACGTACTCTCCTTCCATGGC |
HAGJ01160617 | Hsp70 (heat shock protein 60) | CCGCCTGGTTAGTCACTTTG | AGTGTTCTCTTGGCCCTCTC |
