Table 3 Expression stability values of 10 candidate reference genes calculated by the Best Keeper.

Rank	Liver		Brain		Skin		Gill		Heart		Blood
Rank	Gene	CV	Gene	CV	Gene	CV	Gene	CV	Gene	CV	Gene	CV
1	RPL19	1.54	RPL13	0.72	RPL13	0.58	RPL19	0.49	RPL19	0.59	RPL19	0.59
2	RPL13	1.61	RPL19	0.83	RPL19	0.67	RPL13	0.66	GAPDH	0.68	RPL13	0.60
3	β-actin	1.64	RPS29	1.09	RPS29	0.71	RPS29	0.68	RPS29	0.88	RPS29	0.68
4	RPS29	1.65	β-actin	1.39	GAPDH	0.84	GAPDH	0.84	β-actin	0.89	GAPDH	0.85
5	GAPDH	1.66	GAPDH	1.74	β-actin	0.98	Saha	1.06	RPL13	1.00	Saha	1.24
6	Saha	2.27	Saha	1.77	Saha	1.09	β-actin	1.23	Saha	1.23	β-actin	1.96
7	α-tubulin	2.38	α-tubulin	2.37	ARBP	1.46	18S	1.99	ARBP	1.98	18S	2.10
8	18S	3.24	18S	3.45	18S	2.27	ARBP	2.41	α-tubulin	2.06	ARBP	2.17
9	ARBP	4.19	28S	4.62	α-tubulin	2.54	28S	2.77	28S	2.31	28S	2.85
10	28S	4.69	ARBP	5.55	28S	3.33	α-tubulin	3.84	18S	2.38	α-tubulin	3.15

The stability of the reference genes is represented by the coefficient of variation (CV), and the most stable gene has the lowest CV value.

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