Figure 2
From: Genomic studies controvert the existence of the CUX1 p75 isoform
CRISPR/Cas9 genomic editing precludes the existence of a p75 CUX1 isoform. (a) CRISPR/Cas9 editing approach to selectively delete p200 in the KG-1 cell line, which only has one copy of CUX1. (b) Immunoblot for CUX1 in KG-1 single cell clones edited with a gRNA targeting exon 4 of CUX1 using the ABE217 antibody. (c) Immunoblot for CUX1 in KG-1 single cell clones edited with a gRNA targeting exon 4 of CUX1 using the B-10-HRP antibody. (d) CRISPR/Cas9 editing approach targeting exon 23 of CUX1 to delete all CUX1 isoforms in the KG-1 cell line. (e) Immunoblot for CUX1 in a KG-1 single cell clone edited with a gRNA targeting exon 23 of CUX1 using the ABE217 antibody (n = 3). (f) Immunoblot for CUX1 in a KG-1 single cell clone edited with a gRNA targeting exon 23 of CUX1 or HPRT using the B-10 antibody (n = 3).
