Figure 3
From: Genomic studies controvert the existence of the CUX1 p75 isoform
CRISPR/Cas9 genomic editing of the predicted intronic p75 transcriptional start site does not eliminate the p75 isoform band. (a) CRISPR/Cas9 editing approach using two gRNAs flanking the predicted intronic p75 TSS. (b) PCR screening strategy for identifying single cell clones with deletion of the p75 putative TSS. PCR products from primer pairs spanning the first cut site or the second cut site in intron 20 indicate the lack of a successful deletion. A PCR product from primers spanning the deletion site indicates a successful deletion. (c) Immunoblot for CUX1 using the B-10 antibody in a KG-1 single cell clone with successful deletion of the p75 TSS (n = 3).
