Figure 5
From: Genomic studies controvert the existence of the CUX1 p75 isoform
No p75 CUX1 is detected at the RNA level in human AML and breast cancer cell lines. (a) Schematic of primers used to detect p75. (b) Table of anticipated PCR products and whether they are unique to the p75 isoform. (c) Reverse-transcriptase PCR products after 30 cycles of PCR spanning the intron 20 region of CUX1 using cDNA reverse-transcribed from mRNA in 6 different cell lines (K562, Kasumi-1, KG-1, T47D, MDA-MB-231 and MCF-7) (n = 3). cDNA from the KG-1 Δp75 #21 cell line was used as a negative control for p75 mRNA expression. GAPDH, p200-specific primers (1 and 4) and CUX1 primers spanning all isoforms (ex23 and 24) were used as positive controls. Gel is cropped for clarity.
