Figure 2
ITC thermograms showing interaction of ART with the MN4 aptamer in buffer containing 20 mM TRIS (pH 7.4), 5 mM KCl, 2.4% (v/v) DMSO, in (A) 140 mM NaCl and (B) 0 mM NaCl. (C) Interaction of 2-deoxyartemisinin with the MN4 aptamer in 20 mM TRIS (pH 7.4), 140 mM NaCl, 5 mM KCl, 1.5% (v/v) DMSO. (D) Quinine titrated into the MN4·ART complex. (E) ART titrated into the MN4·quinine complex. Unless otherwise specified, ITC competition experiments were acquired in buffer containing 20 mM TRIS (pH 7.4), 140 mM NaCl, 5 mM KCl, 1% DMSO. All ITC data were acquired at 15 °C.
