Figure 4
(A) 1H NMR spectra showing the imino proton resonances of MN4 as ART is titrated into the sample up to a 1:1.1 molar ratio of aptamer to ligand. Sample contained 1.4 mM aptamer, 20 mM HxNayPO4, pH 7.4, 10% D2O. Final titration point contains ~ 3% DMSO-d6. Spectra acquired at 5 °C. (B) Histograms showing the absolute value of the difference in peak position of the imino protons for free and ligand-bound MN4. Data is normalized to the largest chemical shift difference value. Displayed ligands are ART (red), quinine (green), cocaine (blue) and amodiaquine (purple). The cocaine, amodiaquine and quinine change in chemical shift data are based on previous published data9,14.
