Figure 5
Analysis of the thermal stability of MN19 free and ligand-bound using UV melting plots. Shown in (A) are the normalized average of UV absorbance values at 260 nm versus temperature for the unbound MN19 (black), ART-bound MN19 (blue), and quinine-bound MN19 (red) as a positive control. Displayed in (B) are the first derivative plots. Dashed lines indicate the obtained Tm points of the aptamer. Data acquired in PBS (pH 7.4), 3% (v/v) acetonitrile. Each data point denotes an average of three experiments with the error ribbons corresponding to one standard deviation.
