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Figure 1

From: Osteonecrosis development by tooth extraction in zoledronate treated mice is inhibited by active vitamin D analogues, anti-inflammatory agents or antibiotics

Figure 1

ED71 or VD3 treatment significantly blocks osteonecrosis development in mice treated with zoledronate. (a) Experimental protocol. In all experiments, 8-week-old female C57BL/6 mice received subcutaneous injection of zoledronate (500 μg/kg) once a week. Vehicle (ethanol: 0.05 µl/kg/day), ED71 or 1,25(OH)2D3 (VD3) (0.05 µg/kg/day) was intraperitoneally injected twice a week for 2 weeks before extraction and afterwards twice a week. (b) Two weeks after the first injection when mice were 10 weeks old, the right first and second molars in mandible were extracted. Six weeks after extraction, mandibles were removed, stained with HE, observed microscopically, and the percent of empty lacunae among all lacunae was evaluated. Scale bars = 100 μm (upper) and 20 μm (lower) panels. (c) Data represents mean relative proportion of empty lacunae among all lacunae in bone ± SD (each with n = 5, ***P < 0.001). Representative data are shown of at least two independent and identical experiments, each with n = 5.

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