Figure 8

Osteonecrosis development is not effectively blocked by teriparatide. C57BL/6 mice were administered zoledronate for 2 weeks. PTH (80 μg/kg) or PBS was intraperitoneally injected twice a week for 2 weeks before extraction and afterwards twice a week. One week after the first injection when mice were 10 weeks old, the right first and second molars in mandible were extracted. Six weeks after extraction, mandibles were removed, stained with HE, observed microscopically (a), and the proportion of empty versus whole lacunae was calculated (b). Scale bars = 100 (upper) or 20 μm (lower panels). Data shows the mean percentage (%) of empty versus whole lacunae ± SD (n = 5 each, NS, not significant). Representative data are shown of at least two independent and identical experiments, each with n = 5.