Figure 3

Diminished CO₂ re-assimilation and autotrophic behaviour of ΔagrD1D2 mutants represented through; (A) Comparison of CO₂ partial pressures of fructose-grown cultures after 200 h in three independent ΔagrD1D2 mutants against the WT (n = 4), ΔagrD1D2 (n = 3), ΔagrD1D2_1 (n = 4), ΔagrD1D2_2 (n = 4) and negative control (n = 1); the negative control represented WT cultures without fructose addition. (B–D) Adaptive growth on 200 kPa CO with fermentation product profiles of WT (Black circles) and ΔagrD1D2 (Green squares) that represent first (A), second (B), and third (C) subcultures of WT (n = 2) and ΔagrD1D2 (n = 2) cultures, growing over a period of 29, 19 and 31 days respectively. Main metabolic products, acetate and ethanol were measured at the end of each fermentation. All error bars indicate standard error of the mean, P values equate to: * ≤ 0.05 of statistical difference to the WT.