Figure 3
From: Systematic identification of NF90 target RNAs by iCLIP analysis
Biotin pulldown assays to assess NF90 binding to target mRNAs. The biotinylated RNAs (heavy black bars) synthesized for each mRNA were incubated with cytoplasmic lysates from HEK-293 cells; after pulldown of proteins associated with the biotinylated RNAs, the presence of NF90 was assessed by western blot analysis. Here, we specifically analyzed the interaction of NF90 with biotinylated RNAs spanning different regions of ZFR mRNA (A), PSMA3 mRNA (B), YWHAH mRNA (C), IRF9 mRNA (D), and IRF3 mRNA (E). Beads (no biotinylated RNA); Input, 20 μg lysate; Neg., biotinylated fragment of BAFF 3'UTR RNA. Immunoblotting image of Input, beads and negative control are from two different gels.
