Figure 4
From: Systematic identification of NF90 target RNAs by iCLIP analysis
NF90 silencing modulates the level of select target mRNAs. HEK-293 cells were transfected to express normal (CTR siRNA) or reduced (NF90 siRNA) NF90 levels; 48 h after transfection, the abundance of selected mRNAs and proteins was measured by RT-qPCR analysis (A) and Western blot analysis (B), respectively. In (B) NF90 and ACTB proteins were also assessed by Western blot analysis to monitor the efficiency of the silencing intervention and identify changes in loading between the samples, respectively. Quantification of the Western blot (right panel) were performed using ImageJ. Data in (A) are the means and standard deviation (+ SD) from at least three independent experiments. *, P < 0.05; **, P < 0.01, ***, P < 0.005. CTRL, siRNA control.
