Figure 5
From: Systematic identification of NF90 target RNAs by iCLIP analysis
Influence of NF90 on the stability of target mRNAs. HEK-293 cells that were processed as described in Fig. 4 to express either normal (Ctrl siRNA) or reduced (NF90 siRNA) levels were used to assess the relative rates of clearance of NF90 target mRNAs by RT-qPCR analysis after treatment with actinomycin D for the indicated times. GAPDH mRNA, a stable transcript, and MYC mRNA, an unstable transcript, were included as controls. The levels of mRNAs were first normalized to 18S mRNA levels and then plotted using GraphPad Prism on semi-logarithmic scales relative to 100%, the abundance of each mRNA before adding actinomycin D. Data are the means and standard deviation (+ SD) from at least three independent experiments. *, P < 0.05; **, P < 0.01, ***, P < 0.005.
