Figure 2
Downregulation of SOSTDC1, DACT2, WIF1 in breast cancer cell lines is associated with methylation. (a) Expression levels of SOSTDC1, DACT2, WIF1 in breast cancer cell lines relative to non-tumorigenic HBL100, analysed by qRT-PCR. The CT values were normalized against the internal control GAPDH. Each sample was run in triplicates and mean ± SD of 2^∆∆CT values were plotted in log scale, (b) MSP results of SOSTDC1, DACT2, WIF1 in breast cancer cell lines, (c) Representative figure of bisulphite gene sequencing of the putative promoter region of DACT2 and WIF1 in MDAMB231 cells using gene specific BSP primers. The DACT2 and WIF1 amplicons were 257 bp and 493 bp respectively. The black symbols (
) in chromatogram represent the methylated CpG sites.
