Figure 1 | Scientific Reports

Figure 1

From: A rapid and affordable point of care test for antibodies against SARS-CoV-2 based on hemagglutination and artificial intelligence interpretation

Figure 1

Recombinant proteins and test principle. (a) Principle of NanoSpike-mediated hemagglutination in the presence of αSpike AB. (b) Schematic presentation of recombinant proteins. S, secretion signal (IFNβ); αGPA-nano, GPA-specific nanobody; L, flexible linker; Spike, Spike-RBD of SARS-CoV-2; S-tag, Tandem-Strep-tag; 2xαSpike-Nano, tandem-fusion construct of two Spike-RBD-binding H11-D4 nanobodies, separated by flexible linker; Hinge-IgG1-FC, dimerizing Hinge-FC moiety of IgG1. (c) SyproRuby-stained SDS PAGE of 3 µg of indicated proteins that were expressed in Expi293F cells and purified by one-step affinity purification. Protein yield from 25 ml of Expi293F-cell cultures were 2 mg (NanoControl), 3.75 mg (NanoSpike), 0.55 mg (NanoNuc) and 1.25 mg (NanoLink). Defined amounts of BSA are shown for comparison.

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