Figure 6
From: Opto-electrical bimodal recording of neural activity in awake head-restrained mice
Bimodal recording of neuronal calcium signalling and LFP in CA1 using a GRIN lens and a silicon probe. (a) Position of a GRIN lens and a silicon probe for bimodal recordings in the CA1 area. (b) Confirmation of the placement of the GRIN lens and the silicon probe. Top: A frame of fluorescence image showing the existence of silicon probe shanks under the GRIN lens (Arrowheads). Bottom: An image of a cresyl violet-stained brain section showing the location of the four shanks of the silicon probe (white dotted circles). Orange dashed line indicates the estimated position of the GRIN lens. (c) Left: A ΔF/F image showing Individual neurons expressing GCaMP6s. Right: Example frames indicating changes in fluorescence during a calcium event for each of four neurons. Colors correspond to colors of dashed circles in the left image. (d) An example of temporally matched traces of LFP and neuronal calcium signals. Raw and band pass-filtered traces (150–250 Hz) are presented in black and blue lines, respectively. The band pass-filtered traces show multiple episodes of sharp wave-associated ripples. Calcium signals from different neurons are presented in different colours. (e) Another example temporally matched traces while the mouse runs. We observed clear theta oscillations (8–12 Hz). Data in this figure were recorded 32 days after implantation.
