Figure 2
Accumulation of free and esterified 12-OPDA and dnOPDA is enhanced in guard cells from the atmyb60-1 mutant. (a) Schematic representation of the JA biosynthetic pathway. Red boxes represent metabolites identified as over-accumulated in the atmyb60-1 mutant compared with the wild type. Genes coding for JA biosynthetic enzymes are indicated by their acronyms (uppercase, italics). Heatmaps indicate relative gene expression in LM-purified Col-0 or atmyb60-1 mesophyll and guard cells (Heatmapper, http://www.heatmapper.ca). Genes upregulated in the mutant as compared with the wild type are highlighted in red (13-LOXs). Mutant alleles employed in the study are highlighted in blue (lowercase, italics). Modified from Acosta and Farmer (2010)4. Abbreviations:[M/D]GDG, mono- or di-galactosyldiacylglycerol; 13-HPOT, 13(S)-hydroperoxy-octadecatrienoic acid; 12,13-EOT, (13S)-12,13-epoxy-octadecatrienoic acid; OPC-8:0, 3-oxo-2(2′-pentenyl)-cyclopentane-1-octanoic acid; OPC-6:0, 3-oxo-2(2′-pentenyl)-cyclopentane-1-hexanoic acid; OPC4:0, 3-oxo-2(2'-pentenyl)-cyclopentane-1-butanoic acid; CoA, Coenzyme A. (b–k), relative amounts of free dnOPDA (b,c), free 12-OPDA (d,e), Ara-A (f,g), Ara-B (h,i) and Ara-D (j,k), in whole leaves or epidermal fractions enriched in guard cells obtained from wild type or atmyb60-1 plants. Note the different scale of the Y-axis between whole leaves and guard cells. "a" indicates significant differences between wild type and atmyb60-1 samples (P < 0.01, t-test).
