Figure 3 | Scientific Reports

Figure 3

From: The AtMYB60 transcription factor regulates stomatal opening by modulating oxylipin synthesis in guard cells

Figure 3

AtMYB60 negatively regulates expression of 13-LOX genes. (a) qPCR analysis of 9-LOXs (LOX1 and LOX5) and 13 LOXs (LOX2, LOX3, LOX4 and LOX6) expression in mesophyll or guard cells laser-microdissected from wild type or atmyb60-1 leaves. Relative gene expression was normalized to the expression of the AtACTIN2 gene. (b–m) Histochemical analysis of GUS activity in Arabidopsis stable transgenic LOXpro:GUS lines in the wild type (Col-0) or atmyb60-1 background. Developing leaves from 15-day-old plants were incubated in the staining solution for 24 h. Scale bars represent 20 µm.

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