Figure 4
Defective stomatal opening in atmyb60-1 is associated with 12-OPDA accumulation in guard cells. (a,b), Stomatal aperture measurements in Col-0 and atmyb60-1 epidermal strips determined after 4 h of treatment with 25 or 100 μM MeJA (a) and with 2 or 20 μM 12-OPDA (b). Stomatal opening is expressed as the width/length ratio of the stomatal pore. (c,d) Stomatal aperture (c) and stomatal conductance (gs) (d) of Col-0, atmyb60-1, aos, aos atmyb60-1, Ws, opr3 and opr3 atmyb60-1 plants. (e,f) stomatal aperture (e) and stomatal conductance (f) of Col-0, atmyb60-1, coi1-1, coi1-1 atmyb60-1, coi1-30 and coi1-30 atmyb60-1 leaves. Stomatal opening values represent the mean ± standard error of three separate experiments (n = 100–150 stomata per genotype per experiment). Stomatal conductance values represent the mean ± standard error of three separate experiments (n = 10 leaves per genotype per experiment). (g–j) qPCR analysis of JAZ10 (g), JAZ12 (h), HSP20 and ZAT12 (j) expression in Col-0, atmyb60-1, aos, coi1-1, coi1-1 atmyb60-1, Ws and opr3 10-day-old seedlings. Relative gene expression was normalized to the expression of the AtACTIN2 gene. "a" indicates statistically significant difference between mutants and their respective wild type (Col-0 or Ws) (P < 0.01, t-test).
